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Display major and minor tick marks for log-scale axis, with optional offset for proper labeling of log2(1+x) with numeric offset.

Log fold axis

Usage

minorLogTicksAxis(
  side = NULL,
  lims = NULL,
  logBase = 2,
  displayBase = 10,
  offset = 0,
  symmetricZero = (offset > 0),
  majorCex = 1,
  minorCex = 0.65,
  doMajor = TRUE,
  doMinor = TRUE,
  doLabels = TRUE,
  doMinorLabels = TRUE,
  asValues = TRUE,
  logAxisType = c("normal", "flip", "pvalue"),
  padj = NULL,
  doFormat = TRUE,
  big.mark = ",",
  scipen = 10,
  minorWhich = c(2, 5),
  logStep = 1,
  cex = 1,
  las = 2,
  col = "black",
  col.ticks = col,
  minorLogTicksData = NULL,
  verbose = FALSE,
  ...
)

logFoldAxis(
  side = NULL,
  lims = NULL,
  logBase = 2,
  displayBase = 2,
  offset = 0,
  symmetricZero = TRUE,
  asValues = TRUE,
  minorWhich = NULL,
  doMinor = TRUE,
  doMinorLabels = NULL,
  scipen = 1,
  ...
)

pvalueAxis(
  side = 2,
  lims = NULL,
  displayBase = 10,
  logBase = 10,
  logAxisType = "pvalue",
  asValues = FALSE,
  doMinor = FALSE,
  doMinorLabels = FALSE,
  scipen = 1,
  ...
)

Arguments

side

integer indicating the axis side, 1=bottom, 2=left, 3=top, 4=right.

lims

NULL or numeric range for which the axis tick marks will be determined. If NULL then the corresponding graphics::par("usr") will be used.

logBase

numeric value indicating the log base units, which will be used similar to how base is used in log(x, base).

displayBase

numeric value indicating the log base units to use when determining the numeric label position. For example, data may be log2 scaled, and yet it is visually intuitive to show log transformed axis units in base 10 units. See examples.

offset

numeric offset used in transforming the numeric data displayed on this axis. For example, a common technique is to transform data using log2(1+x) which adds 1 to values prior to the log2 transformation. In this case, offset=1, which ensures the axis labels exactly match the initial numeric value prior to the log2 transform.

symmetricZero

logical indicating whether numeric values are symmetric around zero. For example, log fold changes should use symmetricZero=TRUE which ensures a log2 value of -2 is labeled -4 to indicate a negative four fold change. If symmetricZero=FALSE a log2 value of -2 would be labeled 0.0625.

majorCex, minorCex

numeric base text size factors, relative to cex=1 for default text size. These factors are applied in addition to existing graphics::par("cex") values, preserving any global text size defined there.

doMajor, doMinor, doLabels, doMinorLabels

logical, default TRUE, whether to display each type of tick and label.

  • doMajor display major ticks, at displayBase positions

  • doMinor display minor ticks at intermediate positions

  • doLabels display any labels

  • doMinorLabels display minor labels

asValues

logical, default TRUE, whether to print the exponentiated value, otherwise FALSE will print the log value.

logAxisType

character string with the type of axis values:

  • "normal": axis values as-is.

  • "flip": inverted axis values, for example where negative values should be displayed as negative log-transformed values.

  • "pvalue": for values transformed as -log10(pvalue)

padj

numeric vector length 2, which is used to position axis labels for the minor and major labels, respectively. For example, padj=c(0,1) will position minor labels just to the left of the tick marks, and major labels just to the right of tick marks. This example is helpful when minor labels bunch up on the right side of each section.

doFormat

logical indicating whether to apply base::format() to format numeric labels.

big.mark, scipen

arguments passed to base::format() when doFormat=TRUE.

minorWhich

integer vector indicating which of the minor tick marks should be labeled. Labels are generally numbered from 2 to displayBase-1. So by default, log 10 units would add minor tick marks and labels to the c(2,5) position. For log2 units only, the second label is defined at 1.5, which shows minor labels at c(3, 6, 12), which are 1.5 * c(2, 4, 8).

logStep

integer the number of log units per "step", typically 1.

cex, col, col.ticks, las

parameters used for axis label size, axis label colors, axis tick mark colors, and label text orientation, respectively.

minorLogTicksData

list object created by running jamba::minorLogTicks(), which allows inspecting and modifying the content for custom control.

verbose

logical indicating whether to print verbose output.

...

Additional arguments are ignored.

Value

list with vectors:

  • majorLabels: character vector of major axis labels

  • majorTicks: numeric vector of major axis tick positions

  • minorLabels: character vector of minor axis labels

  • minorTicks: numeric vector of minor axis tick positions

  • allLabelsDF: data.frame containing all axis tick positions and corresponding labels.

Details

This function displays log units on the axis of an existing base R plot. It calls jamba::minorLogTicks() which calculates appropriate tick and label positions.

Note: This function assumes the axis values have already been log-transformed. Make sure to adjust the offset to reflect the method of log-transformation, for example:

  • log2(1+x) would require logBase=2 and offset=1 in order to represent values properly at or near zero.

  • log(0.5+x) would require logBase=exp(1) and offset=0.5.

  • log10(x) would require logBase=10 and offset=0.

The defaults logBase=2 and displayBase=10 assume data has been log2-transformed, and displays tick marks using the common base of 10. To display tick marks at two-fold intervals, use displayBase=2.

This function was motivated in order to label log-transformed data properly in some special cases, like using log2(1+x) where the resulting values are shifted "off by one" using standard log-scaled axis tick marks and labels.

For log fold changes, set symmetricZero=TRUE, which will create negative log scaled fold change values as needed for negative values. For example, this option would label a logBase=2 value of -2 as -4 and not as 0.25.

Note that by default, whenever offset > 0 the argument symmetricZero=TRUE is also defined, since a negative value in that scenario has little meaning. This behavior can be turned off by setting symmetricZero=FALSE.

Examples

plotPolygonDensity(0:100, breaks=100);


plotPolygonDensity(0:100, breaks=50, log="x",
   main="plotPolygonDensity() uses minorLogTicksAxis()",
   xlab="x (log-scaled)");


plotPolygonDensity(log2(1+0:100), breaks=50,
   main="manually called minorLogTicksAxis(logBase=2)",
   xaxt="n",
   xlab="x (log-scaled)");
minorLogTicksAxis(1, offset=1, logBase=2);


plotPolygonDensity(log10(1+0:100), breaks=50,
   main="manually called minorLogTicksAxis(logBase=10)",
   xaxt="n",
   xlab="x (log-scaled)");
minorLogTicksAxis(1, offset=1, logBase=10);


# example with log fold axes
k <- c(-5:5)
plot(x=k, y=k, xaxt="n", yaxt="n",
   xlab="log2 base, displaying tick marks with log10 intervals",
   ylab="log2 base, displaying tick marks with log2 intervals")
axis(3, las=2)
axis(4, las=2)
lfax <- logFoldAxis(side=1, logBase=2, displayBase=2)
lfay <- logFoldAxis(side=2, logBase=2, displayBase=10)
# optionally add x-axis ablines
abline(v=lfax$allTicks, lty="dotted", col="grey88")
abline(v=lfax$majorTicks, lty="dashed", col="grey82")
# optionally add y-axis ablines
abline(h=lfay$allTicks, lty="dotted", col="grey88")
abline(h=lfay$majorTicks, lty="dashed", col="grey82")


# example showing volcano plot features
set.seed(123);
n <- 1000;
vdf <- data.frame(lfc=rnorm(n) * 2)
vdf$`-log10 (padj)` <- abs(vdf$lfc) * abs(rnorm(n))
plotSmoothScatter(vdf, xaxt="n", yaxt="n", xlab="Fold change",
   main="Volcano plot\ndisplayBase=2")
logFoldAxis(1)
pvalueAxis(2)


plotSmoothScatter(vdf, xaxt="n", yaxt="n", xlab="Fold change",
   main="Volcano plot\ndisplayBase=10")
logFoldAxis(1, displayBase=10)
pvalueAxis(2)